June 21, 2019
Bulletin interne de l'Institut Pasteur
Two years ago, the Center for Translational Science was pleased to announce that Simoa technology for quantification of low protein concentrations in biological samples had become available at the Institut Pasteur.
Simoa, Single Molecule Array, is based upon the isolation of individual immunocomplexes on paramagnetic beads using standard ELISA reagents. In contrast to conventional immunoassays, it traps single molecules in femtoliter-sized wells, allowing for a “digital” readout.
This year, a mini symposium will be held on June 27 from 10 to 11.30am in the Jacques Monod lecture hall. A series of scientific presentations will showcase the latest developments related to this technology.
Agenda:
10 – 10.10am: Introduction to Simoa technology – Paula Perin, Quanterix
10.10 – 10.30am: Characterizing type I interferon responses in infection and autoimmune disease – Vincent Bondet, Institut Pasteur
10.30 – 10.50am: Set-up of clinical SIMOA dried blood/CSF spot assays in the Neurology field – Sylvain 10.50 – 11.10am: Single molecule analyses: Opportunities and challenges for better understanding HIV/SIV pathogenesis – Caroline Pereira Bittencourt Passaes, Institut Pasteur
11.10 – 11.30am: The use of SiMOA technology for subpicomolar detection of GAD65 antigen as indicator of pancreatic beta cell death – Katrin Verhaeghen, Universitair Ziekenhuis Brussel (UZ Brussel)
11.30am – 1.30pm: Lunch
1.30 – 2pm: Lab tour, HD-1 and SP-X instrument presentation