Second practical course: image processing for electron microscopy – tomography, October 3 to 7

The second Institut Pasteur practical course on Image Processing for Electron Microscopy focused on Tomography will be held over 5 days, from October 3-7, 2022.

Electron tomography combined with image processing is one of the most powerful methods for studying cellular details in 3D. In situ studies can reveal additional important information about macromolecules in their complex environment and adopted conformations.

This course aims to introduce basic concepts in image analysis and practical experience in tomographic reconstructions for different needs: processing of low-dose tilt series with and without fiducials for 3D volume annotation or subtomogram averaging. Initial steps of subtomogram averaging will also be covered.

The course is aimed at PhD students, post-doctoral fellows, engineers and researchers who have some experience in EM and would like to use electron tomography in the near future. Applicants who already have tilt-series for processing will be prioritized. Participants will become autonomous and able to apply what they have learnt in their projects.

Online registration

Attendees: 12

Application deadline: July 22, 2022

Organizers: Anastasia Gazi, François Bontems and Anna Sartori-Rupp