May 29, 2020
Bulletin interne de l'Institut Pasteur
LuLISA project, bioluminescence as a tool for human diagnostics, from allergy to Covid19
The LuLISA (Luciferase-Linked ImmunoSorbent Assay) research project aims developing high throughput serological tests for epidemiological studies at local, regional or nation scales. In a recent scientific publication, concerning the detection and dosing of IgE specific to several allergens in patients’ blood samples, the use of LuLISA proves to be a vastly improved detection method in comparison with the commercially available kits. The LuLISA has also been adapted to detect various human immunoglobulin types targeting SARS-CoV-2 coronavirus proteins, and it is currently being used as one of the reference methods to evaluate the progress of collective immunity in Covid-19 in France, in collaboration with Santé publique France and several hospitals. This project involves scientists from the Institut Pasteur (Dept. of Immunology and Structural Biology and Chemistry), the CNRS (UMR3523) and the Inserm (U1221, U1222 and UMR1043) in the design of an original assay related to an ELISA (Enzyme-Linked ImmunoSorbent Assay).