New cytometry equipment at Bioaster

Bioaster (lien vers site Bioaster) recently acquired a BD Influx^® cell sorter that has been adapted to perform sorting experiments in controlled gas conditions. This equipment can be used for collaborative projects, it is available on the campus (Building 27 François Jacob, 3rd floor).

The sorting chamber has been covered with a glove box that is continuously fed with nitrogen, thus allowing decreasing O2 concentration in the box below 0.7%. Depending on specific applications requirements, oxygen in the sheath fluid can be depleted, with nitrogen being then used instead of air for pressurization. This provides additional protection of cells and/or micro-organisms towards oxygen exposure during analysis and sorting. An anaerobic chamber has been installed close to the cell sorter to allow handling and preparation of samples to be sorted.

The cell sorter is equipped with three lasers (406 nm/100 mW, 488nm/200 mW and 640nm/120 mW) and has two dedicated sample lines: one for bacteria, the other for eukaryotic cells.
 
For more information, please contact Bioaster's team:

• Vincent Thomas, Head of microbiota program: vincent.thomas@bioaster.org

• Samuel Bellais, Researcher: Samuel.Bellais@bioaster.org